Recombinant DNA Technology

  1. Introduction to RDT
  2. General laboratory practices in RDT
  3. Instrumentation: Principle and Handling
  4. Reagent preparation
  5. Plasmid DNA isolation
  6. Visualization of Plasmid DNA
  7. Quantification Plasmid DNA
  8. Extraction and purification of Plasmid
  9. Polymerize chain reaction
  10. Restriction fragment length analysis (RFLP)
  11. Preparation of Recombinant DNA Molecule
  12. Ligation
  13. Transformation
  14. Competent cell preparation
  15. Transformation
  16. Screening of recombinant DNA molecule